CAS:101182-25-6;(6R,​7R)​-3-​(Chloromethyl)​-​8-​oxo-​7-​[[2-​(2-​thienyl)​acetyl]​amino]​-5-​thia-​1-​azabicyclo[4.2.0]​oct-​2-​ene-​2-​carboxylic Acid (4-​Methoxyphenyl)​methyl Ester

产品名称：CAS:101182-25-6;(6R,​7R)​-3-​(Chloromethyl)​-​8-​oxo-​7-​[[2-​(2-​thienyl)​acetyl]​amino]​-5-​thia-​1-​azabicyclo[4.2.0]​oct-​2-​ene-​2-​carboxylic Acid (4-​Methoxyphenyl)​methyl Ester

产品描述：

qiyueC587843

Chemical Name:(6R,​7R)​-3-​(Chloromethyl)​-​8-​oxo-​7-​[[2-​(2-​thienyl)​acetyl]​amino]​-5-​thia-​1-​azabicyclo[4.2.0]​oct-​2-​ene-​2-​carboxylic Acid (4-​Methoxyphenyl)​methyl Ester

Synonyms
(6R-trans)--3-​(Chloromethyl)​-​8-​oxo-​7-​[[2-​(2-​thienyl)​acetyl]​amino]​-5-​thia-​1-​azabicyclo[4.2.0]​oct-​2-​ene-​2-​carboxylic Acid (4-​Methoxyphenyl)​methyl Ester; (6R,7R)-4-Methoxybenzyl 3-(Chloromethyl)-8-oxo-7-(2-(thiophen-2-yl)acetamido)-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylate

CAS Number : 101182-25-6

Molecular Formula:C₂₂H₂₁ClN₂O₅S₂

Appearance :Off White Solid

Molecular Weight :493

Solubility :Dichloromethane

Category :Standards; Miscellaneous;

Applications
(6R,​7R)​-3-​(Chloromethyl)​-​8-​oxo-​7-​[[2-​(2-​thienyl)​acetyl]​amino]​-5-​thia-​1-​azabicyclo[4.2.0]​oct-​2-​ene-​2-​carboxylic Acid (4-​Methoxyphenyl)​methyl Ester is an intermediate in synthesizing Nitrocefin (N493815), a chromogenic b-lactamase substrate that undergoes colour change from yellow to red as the amide bond in the b-Lactam ring is hydrolyzed by b-lactamase. Nitrocefin undergoes colour changes induced by lactamases produced by Gram-positive and Gram-negative bacteria. Several studies have utilized the colour changing properties of Nitrocefin for the detection of b-lactamase activity from bacterial cell extracts by isoelectric focusing and spectroscopy. Nitrocefin has also been used in studies involving b-lactamase resistant antibiotics.

References
Yang, H., et al.: Biochem., 51, 3839 (2012); El, Z., et al.: J. Pure. Applied. Microbiol, 5, 511 (2011); Nguyen, H.M., et al.: J. Chem. Info. Modeling., 51, 3226 (2011)
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