Oxaliplatin
产品名称：Oxaliplatin
产品描述：
| 产品描述 | Oxaliplatin inhibits DNA synthesis by conforming DNA adducts. |
| 体外活性 | Oxaliplatin有效作用于脑内移植的L1210白血病,MA 16-C移植瘤,B16黑色素瘤移植瘤,Lewis肺癌移植瘤和C26 结肠癌移植瘤.对携带肝癌HCCLM3肿瘤的裸鼠每周腹腔注射10 mg/kg Oxaliplatin,可以明显降低肿瘤体积和凋亡指数.Oxaliplatin作用于小鼠,诱导退行性神经转导降低. |
| 体内活性 | Oxaliplatin有效抑制膀胱癌细胞系RT4和TCCSUP,卵巢癌细胞系A2780,结肠癌细胞系HT-29,胶质母细胞瘤细胞系U-373 mg和U-87 mg和黑色素瘤细胞株SK-MEL-2和HT-144（IC50分别为11 μM, 15 μM, 0.17 μM, 0.97 μM, 2.95 μM, 17.6 μM, 30.9 μM 和 7.85 μM）。Oxaliplatin还能有效抑制人类黑色素瘤细胞系C32和G361（IC50分别为0.98 mM 和0.14 mM）。 |
| 激酶实验 | Binding experiments of electrophysiology: CHO cells expressing the subunit of the voltage-dependent L-type Ca2+ channel are cultrured in medium without serum in the presence of different concentrations of Nisoldipine. Then Ca2+ channel current elicited from a holding potential of -100 mV or -50 mV is recorded at room temperature with the whole-cell configuration of the patch-clamp method using the List EPC-7 patch-clamp amplifer and pClamp software. The concentration of competitor inhibiting 50% of the specific binding represents IC50. |
| 细胞实验 | The cytotoxicity studies are carried out with the sulforhodamine-B microculture colorimetrie assay. Typically, cells are plated into 96-well plates on day 0 and exposed to Oxaliplatin on day 1; the sulforhodamine-B assay is carried out 48 h after Oxaliplatin exposure. The plates are incubated at 37 °C in 5% CO2 and 100% relative humidity at all times except when adding Oxaliplatin and during the final assay period. The initial number of cells plated for the assay ranged from 2-20 × 103 cells/50 /nL/well. The numbers of cells for plating and the drug exposure time are based on pilot studies using the criteria that (a) the cells in control wells are still in the log phase of growth on the day of the assay; (b) the maximum absorbance for the untreated controls on the day of the assay is in the range of 1.0 to 1.5; and (c) cells go through >2 doublings during the drug exposure. Eight wells are used per concentration. The plates are read at 570 and/or 540 nm using a Biotek Instruments model EL309 microplate reader interfaced with an IBM PC-compatible computer. The data are transferred and transformed into a LOTUS 1-2-3 format by the computer program DATALOG, and survival fractions are calculated by comparing the drug treated with control(Only for Reference) |
| 别名 | L-OHP, 奥沙利铂 |
| 化合物与蛋白结合的复合物 |
NMR Solution Structure of an Oxaliplatin 1,2-d(GG) Intrastrand Cross-Link in a DNA Dodecamer Duplex |
| 分子量 | 397.29 |
| 分子式 | C8H14N2O4Pt |
| CAS No. | 61825-94-3 |
存储
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
Ethanol: < 1 mg/mL (insoluble)
DMSO: 20 mg/mL(50.3 mM),DMSO can inactivate Oxaliplatin's activity
H2O: 3 mg/mL (7.55 mM),ultrasonic and warming and heat to 60°C
DMF: 1.67 mg/mL (4.20 mM),Need ultrasonic
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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西安齐岳生物科技有限公司是集化学科研和定制与一体的高科技化学公司。业务范围包括化学试剂和产品的研发、生产、销售等。涉及产品为通用试剂的分销、非通用试剂的定制与研发，涵盖生物科技、化学品、中间体和化工材料等领域。
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