GW 4064
作者：齐岳
发布时间：2023-09-13
17:13:44
点击数：
产品名称：GW 4064
产品描述：
| 产品描述 | GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM). |
| 靶点活性 | FXR:65 nM(EC50) |
| 体外活性 | 在Fisher大鼠中,口服给药GW 4064（ED50 = 20 mg/kg）,能够降低大鼠血清中的甘油三酯. |
| 体内活性 | 在小鼠FXR表达载体转染的CV-1细胞中（EC50=80 nM）,在人FXR表达载体转染的CV-1细胞中（EC50=90 nM）,GW 4064作为选择性非甾体FXR激动剂,能够发挥激动作用。 |
| 细胞实验 | GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis[2]. |
| 分子量 | 542.84 |
| 分子式 | C28H22Cl3NO4 |
| CAS No. | 278779-30-9 |
存储
| Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: 54.3 mg/mL (100 mM)
Ethanol: 5.4 mg/mL (10 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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