Bay 11-7085
作者：齐岳
发布时间：2023-09-13
17:01:54
点击数：
产品名称：Bay 11-7085
产品描述：
| 产品描述 | BAY 11-7085 can irreversibly inhibit the IκBα phosphorylation induced by TNFα (IC50: 10 μM). |
| 靶点活性 | IκBα phosphorylation:10 μM |
| 体外活性 | 在含Caov-3细胞的无胸腺裸鼠体内,BAY 11-7085可使紫杉醇对腹内传播和腹水产生的抑制作用增强.BAY11-7085对脑膜炎相关的NF-κB活性增加有抑制作用,改善被感染大鼠临床状态,并使脑膜炎相关的CNS并发症和脑膜炎症显著衰减. |
| 体内活性 | BAY 11-7085与紫杉醇联用可增强对NF-κB活性的抑制,并使经紫杉醇处理细胞的存活率降低。通过抑制NF-κB,BAY11-7085可抑制TNFα诱导的粘附分子E-selectin,ICAM-1及VCAM-1的表达。BAY11-7085（10 μM）对HUVEC细胞无细胞毒性。此外,Bay11-7085与LY294002联用可协同影响PEL细胞的凋亡。 |
| 激酶实验 | In Gel Kinase Assay: In gel kinase assay for the proteins that phosphorylate IκB-α is carried out as detailed below. Whole cell extracts are prepared from HUVEC treated with TNFα (100 units/ml) for 15 min in the presence or absence of inhibitor (20 μM, pretreatment for 1 h) as indicated. Proteins are separated on a 10% SDS gel containing 0.5 mg/ml HIS-IκB-α. Gels are washed two times in 20% propanol, 50 mM Hepes, pH 7.6, for 30 min and two times in buffer A (50 mM Hepes, pH 7.6, 5 mM 2-mercaptoethanol) for 30 min, followed by a 1-h incubation with buffer A containing 6 M urea, 1 h each in 3, 1.5, and 0.75 M urea in buffer A and 0.05% Tween 20 and 1 h in buffer A with 0.05% Tween 20. The kinase assay is carried out for 1 h at 30?°C in the presence of 50 μM ATP, 5 μCi/ml [32P]ATP, 20 mM Hepes, pH 7.6, 20 mM MgCl2, 20 mM β-glycerophosphate, 20 mM p-nitrophenyl phosphate, 1 mM sodium vanadate, 2 mM dithiothreitol. The gel is washed with 5% trichloroacetic acid and 1% sodium pyrophosphate, dried, and exposed to film. A separate gel with no HIS-IκB-α is assayed as a control. |
| 细胞实验 | Human umbilical vein endothelial cells (HUVEC) are isolated and maintained in culture. Cell toxicity is assessed by morphology and by MTT assay.(Only for Reference) |
| 别名 | BAY 11-7083 |
| 分子量 | 249.33 |
| 分子式 | C13H15NO2S |
| CAS No. | 196309-76-9 |
存储
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
溶解度
DMSO: 24.9 mg/mL (100 mM)
Ethanol: 24.9 mg/mL (100 mM)
( < 1 mg/mL refers to the product slightly soluble or insoluble )
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西安齐岳生物科技有限公司是集化学科研和定制与一体的高科技化学公司。业务范围包括化学试剂和产品的研发、生产、销售等。涉及产品为通用试剂的分销、非通用试剂的定制与研发，涵盖生物科技、化学品、中间体和化工材料等领域。
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